Phosphodiesterase II Product Information

Source: Bovine Spleen


Spleen exonuclease (Phosphodiesterase II) excises 3'-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique.

Unit Definition: One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37°C, pH 6.5, with an RNA substrate.

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DescriptionActivityCodeSizeCat. No.Price
Phosphodiesterase II
Prepared from the 1mM sodium pyrophosphate, pH 6.9, alumina gel eluate of Hilmoe: Biochem. Prep VIII (Meister, A., ed.), John Wiley & Sons, NY, NY, p. 105 (1961).
Store at -20°C.
≥1.2 u/mg dw SPH 10 un LS003603 $105.00 Buy Now
25 un LS003602 $233.00 Buy Now
Bulk LS003600 Inquire

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