Nuclease, S1 Product Information

Source: Aspergillus oryzae


Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.

Stability/Storage: For long term storage in solution, for up to six months, dilute NUCSI to ≥6000 u/ml in water and freeze in aliquots. Dilute solutions can be stabilized by adding 0.1% albumin (Worthington Code: BSANF) and 10% glycerol.

Unit Definition: One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.

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DescriptionActivityCodeSizeCat. No.Price
Nuclease, S1
Source: Aspergillus oryzae
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Store at -20°C.
≥100,000 to 500,000 units per ml SINUC 10 ku LS04070 $49.00 Buy Now
50 ku LS04072 $141.00 Buy Now
Bulk LS04073 Inquire

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