Deoxyribonuclease II Product Information

Source: Porcine Spleen


Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.

Unit Definition: One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.

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DescriptionActivityCodeSizeCat. No.Price
Deoxyribonuclease II
A dialyzed, lyophilized powder.
Store at 2-8C.
≥800 units per mg dry weight HDA 80 ku LS002425 $74.00 Buy Now
Bulk LS002427 Inquire
Deoxyribonuclease II, Purified
Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966). A dialyzed, lyophilized powder.
Store at -20C.
≥12,000 units per mg protein HDAC 20 ku LS005410 $231.00 Buy Now
Bulk LS005411 Inquire
Deoxyribonuclease II, Purified, Solution
Chromatographically prepared. A solution in 50% glycerol.
Store at -20C.
≥12,000 units per mg protein HDACS 2 ku LS005416 $49.00 Buy Now
5 ku LS005418 $112.00 Buy Now
Bulk LS005420 Inquire

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